Vascularized tissue-engineered adipose established via the adipose-derived stem cells-attached scaffolds encapsulated in muscular fasciae with axial pattern blood vessel pedicle

doi:10.3969/j.issn.2095-4344.2013.18.017

Abstract

Abstract:

BACKGROUND: Revascularization mechanism is the decisive factor for the successful construction of tissue-engineered adipose tissue.
OBJECTIVE: To observe the difference of in vivo adipogenic efficacy of adipose-derived stem cells and collagen protein scaffold encapsulated in three muscular fasciae.
METHODS: The rabbit right vascularized latissimus dorsi muscular fasciae was separated and encapsulated with type Ⅰ collagen protein and adipogenic differentiated adipose-derived stem cells complexes as the differentiation group with axial pattern blood vessel pedicle. The rabbit left vascularized latissimus dorsi muscular fasciae was separated and encapsulated with type Ⅰ collagen protein and undifferentiated adipose-derived stem cells complexes as the undifferentiation group with axial pattern blood vessel pedicle. The rabbit gluteus maximus muscular fasciae without specific vascular pedicle was separated and encapsulated with type Ⅰ collagen protein and undifferentiated adipose-derived stem cells complexes as the control group.
RESULTS AND CONCLUSION: Eight weeks after transplantation, hematoxylin-eosin staining and immunohistochemical staining showed that there was new adipose tissue formation in all groups. The mean humid weight of the neonatal fat tissue and the microvessel density in the differentiation group was higher than those in the undifferentiation group and the control group (P < 0.01, P < 0.05). It indicated that the vascularized tissue-engineered adipose was successfully established via adipose-derived stem cells-attached scaffolds encapsulated in muscular fasciae with axial pattern blood vessel pedicle with good in vivo adipogenic efficiency and strong ability to promote angiogenesis.

Key words: organ transplantation, tissue transplantation, clinical application of organ transplantation, adipose-derived stem cells, adipogenic differentiation, latissimus dorsi muscular fasciae, gluteus maximus muscular fasciae, tissue-engineered adipose tissue, adipose tissue, adipose cells, angiogenesis, cell transplantation, collagen scaffold, rabbits, tissue engineering, provincial grants-supported paper

CLC Number:

R318

Wang He-geng1, Li Hong-mian1, Cui Shi-en2, Xu Kun-ming1. Vascularized tissue-engineered adipose established via the adipose-derived stem cells-attached scaffolds encapsulated in muscular fasciae with axial pattern blood vessel pedicle[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(18): 3349-3357.

Figures/Tables 4

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